fluorescent conjugated secondary antibodies (Jackson Immuno)
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Fluorescent Conjugated Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+secondary+antibodies/Cy+5+AffiniPure+Donkey+Anti-Mouse+IgG/pm42529802-336-5-9
Average 96 stars, based on 704 article reviews
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Incubation:Article Title: Generation of Primordial Germ Cell-like Cells from hESCs Using BMP4 and hAFSC-Conditioned Medium. Article Snippet: For detection of SSEA-4, incubate samples (hAFSC) with the primary anti–SSEA4 antibody [MC813-70] (Abcam, Cat. #ab16287; 1:200 dilution) diluted in blocking solution, overnight at 4 ◦C. .. After incubation with the appropriate primary antibody, wash samples three times with PBS and incubate with the appropriate Article Title: Neuroligin-3 interaction with CSPG4 regulates normal and malignant glial precursors through PIEZO1 Article Snippet: Cells were blocked and permeabilized in 3% normal donkey serum and 0.5% triton-x 100 for 30 minutes at room temperature before incubation with 1:1000 dilutions of anti-MBP (Abcam ab7349), CSPG4 (gift from Dr. Jacqueline Trotter), and OLIG2 (R&D AF2418) overnight at 4C in 1% normal donkey serum and 0.3% triton-x 100. .. Slips were washed 3 times in TBS for 5 minutes at room temperature before being incubated with 1:500 of fluorescent Article Title: Acute Cold Exposure Cell-Autonomously Reduces mTORC1 Signaling and Protein Synthesis Independent of AMPK. Article Snippet: Primary antibodies [Puromycin (Millipore MABE343, Bedford, MA, USA), phospho-ACC (Cell Signaling #3661, Danvers, MA, USA), ACC (Cell Signaling #3676), phospho-S6K (Cell Signaling #9234), S6K (Cell Signaling #2708), phospho-S6 (Cell Signaling #4858), S6 (Cell Signaling #2217)] were applied for either 4 h at room temperature or overnight at 4 ◦C. .. Membranes were then incubated in near-infrared Article Title: Acute Cold Exposure Cell-Autonomously Reduces mTORC1 Signaling and Protein Synthesis Independent of AMPK Article Snippet: Primary antibodies [Puromycin (Millipore MABE343, Bedford, MA, USA), phospho-ACC (Cell Signaling #3661, Danvers, MA, USA), ACC (Cell Signaling #3676), phospho-S6K (Cell Signaling #9234), S6K (Cell Signaling #2708), phospho-S6 (Cell Signaling #4858), S6 (Cell Signaling #2217)] were applied for either 4 h at room temperature or overnight at 4 °C. .. Membranes were then incubated in near-infrared Article Title: Emerging role of N6-methyladenosine (m6A) epitranscriptomic changes in adult anxiety after adolescent alcohol exposure. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Competing Programs Shape Cortical Sensorimotor-Association Axis Development Article Snippet: .. Slices were incubated in the appropriate Staining:Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and Imaging:Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and Microscopy:Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and Blocking Assay:Article Title: Competing Programs Shape Cortical Sensorimotor-Association Axis Development Article Snippet: .. Slices were incubated in the appropriate |
