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fluorescent conjugated secondary antibodies  (Jackson Immuno)


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    Structured Review

    Jackson Immuno fluorescent conjugated secondary antibodies
    Fluorescent Conjugated Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+secondary+antibodies/Cy+5+AffiniPure+Donkey+Anti-Mouse+IgG/pm42529802-336-5-9
    Average 96 stars, based on 704 article reviews
    fluorescent conjugated secondary antibodies - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Generation of Primordial Germ Cell-like Cells from hESCs Using BMP4 and hAFSC-Conditioned Medium.
    Article Snippet: For detection of SSEA-4, incubate samples (hAFSC) with the primary anti–SSEA4 antibody [MC813-70] (Abcam, Cat. #ab16287; 1:200 dilution) diluted in blocking solution, overnight at 4 ◦C. .. After incubation with the appropriate primary antibody, wash samples three times with PBS and incubate with the appropriate fluorescent secondary antibodies (CyTM2 IgG Fraction Monoclonal Mouse Anti-Rabbit (Jacksonimmuno; Cat.# 211- 222-171) for DDX4/VASA and DAZL or CyTM3 AffiniPure® Goat Anti-Mouse IgG (H + L) (Jacksonimmuno; Cat.# 115-165-003) for SSEA-4) for 1 h at room temperature in the dark. ..

    Article Title: Neuroligin-3 interaction with CSPG4 regulates normal and malignant glial precursors through PIEZO1
    Article Snippet: Cells were blocked and permeabilized in 3% normal donkey serum and 0.5% triton-x 100 for 30 minutes at room temperature before incubation with 1:1000 dilutions of anti-MBP (Abcam ab7349), CSPG4 (gift from Dr. Jacqueline Trotter), and OLIG2 (R&D AF2418) overnight at 4C in 1% normal donkey serum and 0.3% triton-x 100. .. Slips were washed 3 times in TBS for 5 minutes at room temperature before being incubated with 1:500 of fluorescent secondary antibodies (Jackson ImmunoResearch) for 1 hour at room temperature. ..

    Article Title: Acute Cold Exposure Cell-Autonomously Reduces mTORC1 Signaling and Protein Synthesis Independent of AMPK.
    Article Snippet: Primary antibodies [Puromycin (Millipore MABE343, Bedford, MA, USA), phospho-ACC (Cell Signaling #3661, Danvers, MA, USA), ACC (Cell Signaling #3676), phospho-S6K (Cell Signaling #9234), S6K (Cell Signaling #2708), phospho-S6 (Cell Signaling #4858), S6 (Cell Signaling #2217)] were applied for either 4 h at room temperature or overnight at 4 ◦C. .. Membranes were then incubated in near-infrared fluorescent secondary antibodies [anti-mouse (Jackson Immunoresearch #115-625-174, West Grove, PA, USA) or anti-rabbit (ThermoFisher #SA535571, Waltham, MA, USA)] for 1 h at room temperature. .. Labeled proteins were visualized using a near-infrared scanner (Licor Odyssey DLx), and resulting band intensities were quantified using ImageStudio 5.2 software (LICORbio, Lincoln, NE, USA)

    Article Title: Acute Cold Exposure Cell-Autonomously Reduces mTORC1 Signaling and Protein Synthesis Independent of AMPK
    Article Snippet: Primary antibodies [Puromycin (Millipore MABE343, Bedford, MA, USA), phospho-ACC (Cell Signaling #3661, Danvers, MA, USA), ACC (Cell Signaling #3676), phospho-S6K (Cell Signaling #9234), S6K (Cell Signaling #2708), phospho-S6 (Cell Signaling #4858), S6 (Cell Signaling #2217)] were applied for either 4 h at room temperature or overnight at 4 °C. .. Membranes were then incubated in near-infrared fluorescent secondary antibodies [anti-mouse (Jackson Immunoresearch #115-625-174, West Grove, PA, USA) or anti-rabbit (ThermoFisher #SA535571, Waltham, MA, USA)] for 1 h at room temperature. .. Labeled proteins were visualized using a near-infrared scanner (Licor Odyssey DLx), and resulting band intensities were quantified using ImageStudio 5.2 software (LICORbio, Lincoln, NE, USA)

    Article Title: Emerging role of N6-methyladenosine (m6A) epitranscriptomic changes in adult anxiety after adolescent alcohol exposure.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Competing Programs Shape Cortical Sensorimotor-Association Axis Development
    Article Snippet: .. Slices were incubated in the appropriate fluorescent secondary antibodies (all raised in donkey, Jackson ImmunoResearch Laboratories) diluted at 1:1000 in blocking solution at 4°C, overnight, and washed with PBST (3 x 10min). ..

    Staining:

    Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity
    Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and fluorescent secondary antibodies (Jackson Immuno: 706-545-148, 711-605-152) at 1:500 dilution, and counterstained with DAPI (SouthernBiotech Cat# 0100-20) before imaging on a confocal microscope (UC San Diego Nikon Imaging Core, Model: Nikon AXR Laser Scanning Confocal, 20x objective, 0.75NA). ..

    Imaging:

    Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity
    Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and fluorescent secondary antibodies (Jackson Immuno: 706-545-148, 711-605-152) at 1:500 dilution, and counterstained with DAPI (SouthernBiotech Cat# 0100-20) before imaging on a confocal microscope (UC San Diego Nikon Imaging Core, Model: Nikon AXR Laser Scanning Confocal, 20x objective, 0.75NA). ..

    Microscopy:

    Article Title: Experience-induced NPAS4 reduces dendritic inhibition from CCK+ inhibitory neurons and enhances plasticity
    Article Snippet: .. Tissue was flash-frozen in OCT, sectioned on a cryostat into 20 μm sections, stained with antibodies against NPAS4 (Rabbit anti NPAS4, Activity Signaling Cat# AS-AB18A-100) at 1:1000 dilution and NeuN (Guinea pig anti NeuN, Synaptic Systems Cat# 266 004) at 1:1000 dilution and fluorescent secondary antibodies (Jackson Immuno: 706-545-148, 711-605-152) at 1:500 dilution, and counterstained with DAPI (SouthernBiotech Cat# 0100-20) before imaging on a confocal microscope (UC San Diego Nikon Imaging Core, Model: Nikon AXR Laser Scanning Confocal, 20x objective, 0.75NA). ..

    Blocking Assay:

    Article Title: Competing Programs Shape Cortical Sensorimotor-Association Axis Development
    Article Snippet: .. Slices were incubated in the appropriate fluorescent secondary antibodies (all raised in donkey, Jackson ImmunoResearch Laboratories) diluted at 1:1000 in blocking solution at 4°C, overnight, and washed with PBST (3 x 10min). ..



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    Image Search Results


    The results of dental calculus mineralization assays. ( A ) Fluorescent stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.

    Journal: Veterinary Sciences

    Article Title: Neutrophil Extracellular Traps Promote the Formation of Canine Dental Calculus

    doi: 10.3390/vetsci13060593

    Figure Lengend Snippet: The results of dental calculus mineralization assays. ( A ) Fluorescent stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.

    Article Snippet: An anti-rabbit fluorescent secondary antibody (AS011, Abclonal, 1:100) was incubated at room temperature for 1 h in the dark.

    Techniques: Control, Concentration Assay